Effect of global or hypodermal depletion of RPOA-2

To investigate the contributions of hypodermal ribosome biogenesis to organism-wide growth, we established C.elegans strains where RPOA-2 can be depleted globally or particularly in hypodermis using auxin-inducible degradation (AID) system. We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different strains at 2 time points. Overall design: Comparative gene expression profiling analysis of RNA-seq data for global RPOA-2 depleted strains (degron::GFP::rpoa-2; eft-3p::TIR1, embryo, +IAA), hypodermal RPOA-2 depleted strains (degron::GFP::rpoa-2; col-10p::TIR1, embryo, +IAA), and control strains (degron::GFP::rpoa-2, embryo, -IAA).