Results of Bayes-Empirical-Bayes analyses identifying positively selected sites.

Sites in vWF that were identified as being under positive selection (>1) with posterior probability >0.50 in Bayes-Emipircal-Bayes analyses with Didelphini (either excluding or including Chironectes) assigned as foreground lineages.1Numbered according to the mature vWF peptide in Mus.2Sites inferred to be under positive selection with P≥0.95 are shown in bold.3Positively selected sites corresponding to those involved in botrocetin binding in Mus are indicated. An additional five sites (Arg629, Arg632, Lys660, Gln661, Lys667) bind botrocetin in Mus but are not inferred to be positively selected in any of our analyses of opossum vWF sequences.