Effects of salinity stress on physiological characteristics and gata gene family analysis of Cynoglossus semilablus

Since the salinity of semi-smooth tongue sole purchased from the farm is 25%, the semi-smooth tongue sole purchased from the farm is selected as the control group and the semi-smooth tongue sole purchased from the high salt group under the condition of 35%. Samples are taken at different periods of salinity stress, and the physiological and biochemical markers are determined by selecting the internal tissues, including the tissue morphology of the slices. Intestinal flora, liver enzyme activity, liver transcriptome, fluorescence quantification of each tissue, etc. Low salt 15%, high salt 35% for the experimental group, salinity 25% for the control group. The changes of physiological and biochemical indexes and target gene expression in tissues of Cynoglossus semilabelis under different salinity stress were analyzed to explore its adaptability to salinity. Through transcriptome sequencing, we can comprehensively understand the changes of gene expression in fish under salinity stress, and provide important information for the study of the salinity adaptation mechanism of fish. The differentially expressed gene families of Cynoglossus semilablus were screened out under salinity stress, and their functions and mechanisms were discussed by bioinformatics analysis. Real-time fluorescence quantitative PCR was used to verify the expression levels of some differentially expressed gata gene families and further determine their role in salinity stress.