LUTI-dependent Downregulation of a Cell Cycle Transcription Factor is Key for Timely Meiotic Entry

We used RNA-seq to monitor expression of early meiotic genes and expression of mitotic transcription factor SBF targets. Increased SBF activity was achieved through either overexpression of SBF unique subunit Swi4 , removal of LUTI-mediated regulation of Swi4 levels, or nuclear depeletion of negative regulator of SBF Whi5. Cells were grown as described in Su et al., 2023. Samples collected at 2 hours in SPO media. 2-3 biological replicates included for each genotype.