AID associated protein LC-MS .csv

To identify the protein(s) affecting the activity of AID, we tried to identify the AID interacting proteins by expressing AID-FLAG in 293T cells. The AID interacting proteins were subject to co-immunoprecipitation with anti-FLAG-conjugated beads and then separated by SDS-PAGE. Multiple silver-stained bands, especially proteins over 100 kDa were observed.The visualized bands were excised and examined by mass spectrometry after trypsin digestion. In total 1194 proteins were identified. Among the interactors, 17 proteins were AID-specific interacting proteins.We were especially interested in the factors involved in PTMs.