Neurological development following single polycyclic aromatic hydrocarbons (PAH) exposure

This study examined the effects of single polycyclic aromatic hydrocarbons (PAHs) exposure on brain and neurological development. Embryos were exposed at 12 hpf for 48 h. After 48 h, survival was assessed and each replicate was split in 2 for imaging. Once fish were imaged, they were used for calcein staining to assess vertebral column development and calcium uptake and distribution. Images were analyzed for the brain, eye, iris, and pericardial area, spine length and total length.