RNA-Seq of normal and infarcted DBA/2J and C57Bl6/N adult female hearts.

We have observed that DBA/2J and C57Bl6/N mice exhibit different responses to permanent coronary artery ligation, with mice in a C57 background having about a 14-fold increase in cardiomyocyte S-phase activity as compared to DBA mice. We mapped the responsible gene to the distal arm of Chromosome 3 in the C57 background. We then RNA-Seq analyses on hearts from normal and infarcted DBA and C57 mice, with the hope of identifying candidate genes within the region of interest on the distal arm of Chromosome 3 which are differentially expressed. These genes identified Tnni3k as a potential candidate contributing to the elevated S-phase phenotype. Overall design: Adult female mice (3 moths old) were subjected to permanent coronary artery ligation and harvested 2 weeks later. Control animals were untreated. Hearts were harvested, and the intraventricular septum and right ventricles were dissected and used to generate RNA for RNA-Seq analyses. 8 DBA/2J mice (4 with ligation and 4 control) and 8 C57Bl6/N mice (4 with ligation and 4 control) were analyzed.