RecBCD Coordinates Repair of Two Ends at a DNA Double-Strand Break, Preventing Aberrant Chromosome Amplification [MFA]. RecBCD Coordinates Repair of Two Ends at a DNA Double-Strand Break, Preventing Aberrant Chromosome Amplification [MFA]

The effect of an induced site-specific DNA double-strand break on DNA abundance across the chromosome of E. coli cells expressing or not RecD protein, was investigated by marker frequency analysis. Overall design: gDNA was isolated from two independent cultures ('biological repeats') of four, exponentially growing E. coli strains. Two strains experienced the induced DNA double-strand break (DL2006 and DL3391), two did not (DL2573 and DL3743). Two strains were wild-type for the recD gene (DL2006 and DL2573), two had the recD gene deleted (DL3391 and DL3743). gDNA was also isolated from two independent stationary phase cultures of strain DL2573. The libraries of the 10 gDNA preps were multiplexed and run 4 seperate times ('sequencing runs')