Development of an LC-MS method for the determination of simvastatin and its hydroxy acid form in muscle tissue

The data describes parameters that were optimized during an LC-MS method validation. The method includes four compounds: simvastatin (SIM), simvastatin hydroxy acid (SIMA), lovastatin (LOV), and lovastatin hydroxy acid (LOVA). LOV and LOVA were used as internal standards, but are presented in the enclosed data.MS scan spectra (both polarization positive/negative) and fragmentation spectra obtained with different collision energies were placed in the compressed folder Spectra.zip.Solvents_for_homogenization_and_extraction.xls: presents signal intensity obtained for four compounds using different homogenization and extraction agents (mean value from three repetitions and RSD).Proportion_for_homogenization_and_extraction.xls: presents signal intensities, average value, standard deviation, and RSD obtained for four compounds during optimization of proportion solvent to tissue at homogenization and extraction process.Moreover, data describing chromatographic parameters optimization are included in the following files:Comparison_mobile_phase.xls: presents peak intensities obtained for four compounds with different mobile phase A compositions.Gradients.zip: show chromatograms obtained with different chromatographic elution (isocratic or gradient) with additional data describing the chromatographic parameters and gradient composition.Files describing calibration curve:Calibration_curves.zip: shows calibration curves plots in three repetitions with the curve equation and R2 value.Calibration_curve.xls: presents data used to build calibration curves presented in zip file.