Expression data from A549 human lung adenocarcinoma cells with and without knockdown against CNOT3. Expression data from A549 human lung adenocarcinoma cells with and without knockdown against CNOT3

The physiological importance of mRNA degradation through the CCR4-NOT deadenylase has recently been highlighted. For example, mutation in CNOT3, a gene coding for sCNOT3 subunit of the CCR4-NOT complex, is found to be associated with T-cell acute lymphoblastic leukemia, T-ALL, though its contribution to other cancers has not been reported. To identify the taget mRNAs of CNOT3 in human non-small cell lung cancer (NSCLC), we performed the microarray analysis using A549 cells with and without knockdown against CNOT3. Overall design: We established A549 cells stably expressing tetracyclin-induible shRNA against non-targeting control (A549-T-shNTC) or CNOT3 targeting two different sequences (A549-T-shCNOT3-1 or A549-T-shCNOT3-2) using a lentiviral system. We evaluated the mRNA expression of each A549 stable with or without Doxycyclin (DOX; 1 ug/ml) treatment for 72 hours.