Hong Ma, Jagath Samarabandu, Recandu Devdhar, Raj Acharya, Ping-chin Cheng, Chunling Meng, Ronald Berezney (2011) CIL:39235, Macropus sp. MG002, kidney cell. CIL. Dataset

Shown is a chromosome spread of a PtK1 cell pulsed with BrdU in early S phase, then treated with colcemid to arrest cells at metaphase. Spreads were immuno-labeled with FITC-conjugated anti-BrdU antibodies and counterstained with propidium iodide (red). Replication is confined to discrete sites of replication, some of which appear to occur in bands (arrows).

本图展示了PtK1细胞在S期早期接受BrdU脉冲处理后，经秋水仙胺处理使细胞停滞于有丝分裂中期的染色体铺展。铺展区域经FITC标记的抗BrdU抗体进行免疫染色，并使用碘化丙啶（红色）进行复染。复制过程局限于特定的复制位点，其中一些位点似乎位于带状区域（箭头所示）。