DNA methylation restricts default deterministic fates towards neural and germline lineages during early development

We investigate the impact of DNA methylation on chromatin accessibility during epiblast differentiation. To this end, we used in vitro Epiblast-like cell (EpiLC) differentiation on WT and DNA-methylation free TKO murine ESCs. We next performed ATAC seq using TDE1 Tn5 transposase (Nextera). ATAC-seq on WT and TKO ESCs (D0), and D2, D4 EpiLCs