LC–MS/MS method for simultaneousestimation of raloxifene, cladrin in ratplasma: application in pharmacokineticstudies – supplementary materials

Aim: A newer LC–MS/MS method was developed and validated for the simultaneous quantification ofraloxifene (RL) and cladrin (CL). Methodology: Both drugs were resolved in RP-18 (4.6 × 50 mm, 5 μ)Xbridge Shield column using acetonitrile and 0.1% aqueous solution of formic acid (FA) (70:30% v/v)as mobile phase by using biological matrices in female Sprague–Dawley rats using–MS/MS. Results: Thedeveloped method was found to be linear over the concentration ranges of 1–600 ng/ml, and lowerlimit of quantification was 1 ng/ml for RL and CL, respectively. Pharmacokinetic results of RL+CL showedCmax = 4.23 ± 0.61, 26.97 ± 1.14 ng/ml, at Tmax(h) 5.5 ± 1.00 and 3.5 ± 1.00, respectively. Conclusion:Pharmacokinetic study results will be useful in the future for the combined delivery of RL and CL forosteoporosis treatment.