Sequencing of chimeric deletion junctions

To investigate the impact of spatial proximity on CRISPR-induced structural variant formation, sgRNAs were designed to target topologically associated domains (TADs) on the chromosomes 2, 6, 10, 17, 21 and X. The sgRNAs were paired to generate two double-strand breaks by CRISPR/Cas9. PCR amplicons from chimeric deletion-junctions were sequenced on the Illumina MiSeq platform (Eurofins Genomics, Germany) to investigate the composition of the ligation sites. Authors: M. Dahl-Jessen, T. Terkelsen, R. O. Bak, and U. B. Jensen.