Global transcriptome analysis identifies genes regulated by FDP

Flowering of Arabidopsis is induced at the shoot meristem by environmental and endogenous signals. FLOWERING LOCUS T (FT) protein is a systemic signal that induces rapid flowering in response to long summer days (LDs) whereas gibberellin (GA) growth regulators trigger flowering by default in short days (SDs). Mutations in the bZIP transcription factor FDP, which physically interacts to FT, cause late flowering and dwarfism. In order to study the genetic pathways affected by FDP, we compared the global transcriptome changes of Col-0 plants carrying a fusion of the strong ubiquitously expressed CaMV35S promoter to an FDP DsRNAi construct (35S::FDP dsRNA). Col-0 wt and 35s::FDP dsRNA plants were grown under LD conditions during 9 days. Aerial parts of each genotypes were harvested at the end of the light period (ZT16). Biological triplicates of each genotype were used for the Tiling Array experiments.