Sequencing of LCMV Clone 13 genomic fragment from the late stage of infection. LCMV Clone 13 late stage of infection

RNA viruses represent a unique model for evolutionary biology, because of extremly high error rate (10^-3 substitution per site), small genomes and large population sizes. Lymphocytic choriomeningitis virus (LCMV) is a prototypic member for Arenaviruses, that serves as a model system for multiple virological studies. This virus infects preferentially Rodents. LCMV genome consists of two single stranded RNA molecules (L - large and S - small segments). MinION provided by Oxford Nanopore Technologies, allows to get a single-molecule resolution, that makes it a valuable tool to reconstruct viral evolution both, in vivo and in vitro. We sequenced a 1.8 Kb fragment of the L segment using MinION (Oxford Nanopore Technologies, Oxford, UK) obtained from the tissue of infected C57BL/6J mouse at the late stage of infection and from the plasmid encoding viral L segment LCMV. This approach could potentially provide us with a more complete understanding of viral evolution process.