Magelona mirabilis µCT scan

µCT scan of a specimen of Magelona mirabilis stained with PTA for one week. Scan and reconstruction were obtained with the following settings: [System] Scanner=SkyScan1272 Instrument S/N=15H09112 Software Version=1.1.10 Source Type=HAMAMATSU_L11871_20 Camera Type=XIMEA xiRAY16 Camera Pixel Size (µm)=7.4 Camera X/Y Ratio=1.0058 [Acquisition] Filename Index Length=8 Number Of Files= 940 Number Of Rows= 1640 Number Of Columns= 2452 Partial Width=OFF Image crop origin X=0 Image crop origin Y=0 Camera binning=2x2 Image Rotation=0.00400 Optical Axis (line)= 932 Camera to Source (mm)=274.64085 Object to Source (mm)=55.67001 Source Voltage (kV)= 60 Source Current (µA)= 166 Image Pixel Size (µm)=2.999977 Scaled Image Pixel Size (µm)=2.999977 Image Format=TIFF Depth (bits)=16 Reference Intensity=57000 Exposure (ms)=2832 Rotation Step (deg)=0.200 Use 360 Rotation=NO Scanning position=2.271 mm Frame Averaging=ON (4) Random Movement=OFF (8) Flat Field Correction=ON FF updating interval=55 Geometrical Correction=ON Filter=Al 0.25mm Gantry direction=CC Rotation Direction=CC Type of Detector Motion=STEP AND SHOOT Scanning Trajectory=ROUND Number Of Horizontal Offset Positions=1 Number of connected scans=3 Current scan number=1 Number of lines to be reconstructed=1093 Scan duration=3h:17m:30s Maximum vertical TS=5.0 [Reconstruction] Reconstruction Program=NRecon Program Version=Version: 1.7.1.0 Reconstruction engine=InstaRecon Engine version=Version: 2.0.3.7 Reconstruction from batch=No Postalignment=9.50 Connected Reconstruction (parts)=3 Sub-scan post alignment [0]=8.500000 Sub-scan post alignment [1]=9.000000 Sub-scan post alignment [2]=9.500000 Sub-scan scan length [0]=1091 Sub-scan scan length [1]=1093 Sub-scan scan length [2]=1093 Used extra shift in X per scan(micron)= 0.000 -1.327 0.089 Used extra shift in Y per scan(micron)= 0.000 -13.707 -21.439 Time and Date=Jul 14, 2021 12:03:18 First Section=315 Last Section=3430 Reconstruction duration per slice (seconds)=0.294737 Total reconstruction time (2185 slices) in seconds=644.000000 Section to Section Step=1 Sections Count=3116 Result File Type=TIF Result File Header Length (bytes)=12 Result Image Width (pixels)=1960 Result Image Height (pixels)=1960 Pixel Size (µm)=2.99998 Reconstruction Angular Range (deg)=188.00 Use 180+=OFF Angular Step (deg)=0.2000 Smoothing=0 Ring Artifact Correction=8 Draw Scales=OFF Object Bigger than FOV=OFF Reconstruction from ROI=ON ROI Top (pixels)=2115 ROI Bottom (pixels)=153 ROI Left (pixels)=194 ROI Right (pixels)=2156 ROI reference length=2452 Filter cutoff relative to Nyquist frequency=100 Filter type=0 Filter type description=Hamming (Alpha=0.54) Undersampling factor=1 Threshold for defect pixel mask (%)=0 Beam Hardening Correction (%)=0 CS Static Rotation (deg)=0.00 Minimum for CS to Image Conversion=-0.039105 Maximum for CS to Image Conversion=0.529521 HU Calibration=OFF BMP LUT=0 Cone-beam Angle Horiz.(deg)=7.559778 Cone-beam Angle Vert.(deg)=5.060351 Automatic matching in Z=50 Automatic matching in X/Y=50 Automatic matching in rotation=0.000000 Automatic fusion=1 Data size has been reduced by conversion from 16-bit to 8-bit and decreasing the scale of the images.