Epigenome analysis in several cohorts of HIV progession and in uninfected individuals

Genome wide DNA methylation profiling of CD4 T cells from uninfected and HIV-infected individuals (viremic, ART-suppressed and elite controllers [EC]) The Illumina Infinium 450k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across approximately 485,577 CpGs in DNA from peripheral CD4 T cells samples. Samples included: 22 from HIV-uninfected individuals (uninfected group), 42 from HIV-infected individuals (21 from HIV-infected viremic (viremic group) and 21 from the same participants after viral suppression (viral load< 50 copies HIV-1 RNA/plasma) by antiretroviral therapy administrarion (ART group), and 21 from elite controllers (EC group) Bisulphite converted DNA from the 85 samples were hybridized to the Illumina Infinium 450k Human Methylation Beadchip v1.2. Table "correspondence_paired_samples.csv" shows correspondence between 21 individuals from HIV-infected viremic (viremic group) and the same 21 individuals after viral suppression.