Psuedouridine sequencing rRNA in Rnmt cKO CD4 T cells

We investigated the role of RNMT in T cells using an Rnmt conditional knockout mouse model. We report that the mRNA cap methyltransferase, RNMT, supports naïve T cell survival and activation-induced proliferation. We demonstrate that RNMT has gene-specific impacts in T cells, selectively regulating expression of terminal polypyrimidine tract (TOP) mRNAs and small RNAs including snoRNAs. To deterine if had any effect on ribosomal RNA modification we carried out pseudoridine sequencing. Overall design: Sequencing of total RNA either CMC treated (to form aducts on pseudouridine) or mock treated from 20 hour activated CD4 T cells from Rnmt fl/fl CD4cre (Rnmt cKO) and Rnmt fl/fl (control) mice.