RNA-seq of tomato leaves under JCK1241 treatment and/or Ralstonia solanacearum challenge

This study performed RNA sequencing (RNA-seq) of tomato (Solanum lycopersicum, cultivar Seokwang) leaves to investigate transcriptional responses under treatment with JCK1241 and/or challenge with the bacterial wilt pathogen Ralstonia solanacearum. A total of 12 samples were analyzed, including control, pathogen-infected, treatment-only, and combined treatment-plus-pathogen conditions Tomato leaves were sampled from cultivar PNU 01 grown under controlled conditions. Four groups were studied: (i) control, (ii) R. solanacearum-infected, (iii) JCK1241-treated, and (iv) JCK1241 treatment plus pathogen infection. Three biological replicates were included per group. RNA was extracted, sequenced using Illumina paired-end sequencing, and analyzed for differential gene expression, annotation, and clustering