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Generation of Locus-Specific Degradable Tag Knock-ins in Mouse and Human Cell Lines

Mendeley Data2021-05-04 更新2026-04-09 收录

基因敲入技术

可降解蛋白标签

数据链接：

https://data.mendeley.com/datasets/mjd8zdmr6h 数据链接链接失效反馈

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资源简介：

DNA sequence of the mouse Kansl3 genomic locus with the knock-in of the BSD-P2A-2xHA-dTAG cassette at the N-terminal end

应用场景：

创建时间：

2021-05-04

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基因敲入技术

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H11位点位于小鼠11#染色体，如果将此基因敲入小鼠与其他小鼠品系配繁，请尽量避免两个靶基因位于同一染色体上，否则将无法得到双基因阳性同时敲入纯合的小鼠后代。 Mx1基因BAC需购买，需要增加周期及费用。需要合成Human GH 序列，需要增加周期及费用。敲入片段约8.3kb，后代小鼠需要安排Southern鉴定，需要增加相应周期。

中国科技资源共享网2026-03-03 更新230

Supplemental Material for Bosch et al., 2019

果蝇遗传学

基因敲入技术

Supplemental data (figures and tables) for the manuscript "Gene knock-ins in Drosophila using homology-independent insertion of universal donor plasmids" accepted at GENETICS

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Supplemental Material for Bosch et al., 2019

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Supplemental data (figures and tables) for the manuscript "Gene knock-ins in Drosophila using homology-independent insertion of universal donor plasmids" accepted at GENETICS

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Sequences used to knock-in the V5 epitope in the Shb gene.

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Sequences used to knock-in the V5 epitope in the Shb gene.

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