Estimating bloodstain age in the short term based on DNA fragment length using nanopore sequencer

We used a nanopore sequencer to quantify DNA fragments > 10,000 bp in size and then evaluated their relationship with short-term bloodstain age. Moreover, DNA degradation was investigated after bloodstains were wetted once with water. Bloodstain samples on cotton gauze were stored at room temperature and low humidity for up to 6 months. Bloodstains stored for 1 day were wetted with nuclease-free water, allowed to dry, and stored at room temperature and low humidity for up to 1 week. The proportion of fragments > 20,000 bp in dry bloodstains tended to decrease over time, particularly for fragments > 50,000 bp in size. This trend was modeled using a power approximation curve, with the highest R2 value (0.6475) noted for fragments > 50,000 bp in size; lower values were recorded for shorter fragments. The proportion of longer fragments was significantly reduced in bloodstains that were dried after being wetted once, and there was significant difference in fragments > 50,000 b..., , , These fastq files were used for analysis in this study.

A file with a \"*\" after it indicates that the files contain DNA CS (DNA control strand; 3.6 kb standard amplicon mapping the 3' end of the Lambda genome) sequences for quality check. The read count percentage calculations were performed after excluding these sequences.

A folder with a \"w\" prefix indicates that the fastq data are from the experiments (figure 2) in which the bloodstain was wetted with water.

\"Alignment summary\" folder includes the results of mapping with hg38 by the MinKNOW software.

Folder PATH listing

|

+---1day

\| AJM454_pass_bc2cd138_0.fastq(*)

\| ALB645_pass_20c53053_0.fastq(*)

\| ALL990_pass_1a2f28eb_0.fastq(*)

\| ANN476_pass_ed34f4ba_46227b6b_0.fastq(*)

|

+---2days

\| ALB394_pass_929d1638_0.fastq(*)

\| ALR682_pass_50fad96d_0.fastq(*)

\| ANN386_pass_6e9ecc23_89da19c2_0.fastq(*)

\| ANR037_pass_7afa0862_06d9d000_0.fastq(*)

|

+---3days

\| AJM...