Rare and widespread: Integrating Bayesian MCMC approaches, Sanger sequencing and Hyb-Seq phylogenomics to reconstruct the origin of the enigmatic Rand Flora genus Camptoloma

Premise

Genera that are widespread but have a geographically discontinuous distribution and are represented by few species are intriguing. Did they achieve their disjunct distribution recently, or is it ancient in origin? Why are they species-poor? The Rand Flora is a continental-scale floristic pattern in which closely related species appear co-distributed in isolated regions over the edges of Africa and nearby archipelagos. Genus Camptoloma (Scrophulariaceae) is the most notable example, comprising three species isolated from each other at the ends of the African continent: C. canariense in the west, endemic to the Canary Islands; C. lyperiiflorum in the east, endemic to the Horn of Africa - Southern Arabia; and C. rotundifolia, restricted to Southern Africa.

Methods

Here, we employed Sanger sequencing of nuclear and plastid markers, together with genomic target sequencing of 2190 low-copy nuclear genes, to infer interspecies relationships and the position of Camptoloma withi...