Smart nanostructures for targeted oxygen-producing photodynamic therapy of skin photoaging and potential mechanism - supplementary data

Background: Photodynamic therapy increases collagen and decreases solar fibrosis in photoaged skin;
however, the efficacy of photodynamic therapy is limited in tissues with a hypoxic microenvironment.
Methods: A novel autogenous oxygen-targeted nanoparticle, named MCZT, was synthesized based on
the zeolitic imidazole framework material ZIF-8, methyl aminolevulinate, catalase and an anti-TRPV1
monoclonal antibody, and its effects on skin photoaging were investigated. Results: MCZT was successfully
synthesized and showed uniform particle size, good dispersion, and excellent biocompatibility and safety.
Moreover, MCZT effectively alleviated UV-induced inflammation, cellular senescence and apoptosis in
HFF-1 cells. In in vivo models, MCZT ameliorated UV-evoked erythema and wrinkling, inflammation and
oxidative stress, as well as the loss of collagen fibers and water, in the skin of mice. Conclusion: These
findings suggest that MCZT holds promising potential for the treatment of skin photoaging.