Single-cell multiome sequencing of human kidney anatomical regions with SHARE-seq

We developed an optimized, low-cost, split-pool barcoding-based multimodal profiling protocol based upon SHARE-seq (concurrent single-cell ATAC/RNA-seq). With SHARE-seq, we profiled human kidney samples from multiple different anatomical regions. Therefore, we develop a large-scale multimodal single-cell atlas for 3D anatomy of the human kidney. We profiled 48 human kidney samples from kidney cortex, medulla, papilla, ureter and renal artery. Sequencing of the SHARE-seq libraries were done on two Novaseq-S4 flowcells and one Novaseq-S2 flowcell.