Amplicon SMF for Pol II occupancy project

Amplicon Single Molecule Footprinting (SMF) data. SMF data is obtained by treating extracted nuclei with a GpC and a CpG methyltransferases, where binding of proteins on DNA, e.g. nucleosomes and RNA Polymerase II (Pol II), leave behind unmethylated cytosines as footprints. Data in this experiment comprises SMF data obtained from DNA methyltransferase triple knockout mouse embryonic stem cells (DNMT TKO mESCs) treated with either 500 nM triptolide for 30 minutes or DMSO as a control.