Using serially collected specimens to investigate the potential population genetic consequences of reported declines in eastern woodland salamanders

This dataset includes all data and scripts used to investigate demographic shifts in Plethodon salamanders. We designed exonic target loci using custom scripts, generated sequence data via exon-based target capture, and called SNPs using SECAPR v.1.1.15. Here, we provide VCF files from our variant calling pipeline. We calculated summary statistics using the populations module in STACKS and estimated Tajima's D using Python libraries. We provide all scripts used to design exonic capture probes, process genomic data, and calculate summary statistics.