Expression data from Human Embrionic Stem Cells (hESC) from three different lines cultured on feeders or Matrigel with and without ROCK Inhibitor Y-27632. Homo sapiens

Microarray expression data from three different cell lines of human embrionic stem cells (Hs181, Hs293, Hs420), grown under feeder and feeder-free conditions (matrigel) with the addition of Y-27632 (ROCK Inhibitor) We assessed the difference between cell lines and the impact of feeder-free vs matrigel conditions upon the addition of the ROCK Inhibitor Y-27632 Overall design: Cells from three different cell lines (Hs181, Hs293, Hs420) were grown for 1 day on the different experimental conditions described: Feeder, Matrigel, and Matrigel + Y-27632, then RNA was extracted