Cancer treatment with radiation-chemotherapy rapidly induces molecular pathways for both resistance and new therapeutic targets. Cancer treatment with radiation-chemotherapy rapidly induces molecular pathways for both resistance and new therapeutic targets

FaDu tumors were implanted into Athymic mice then treated with HSP90 inhibitor Onalespib (5 days per week, 55mg/kg for 2 weeks), radiation (3 x 2 Gy for 1 week) or combination (2 week Onalespib, 1 week radiation concurrent) The purpose of this study was to understand short term (1 week and 2 week post treatment) gene changes in FaDu tumors that had been injected into hind limbs of athymic mice. A drug treatment, a radiation treatment, a combination treatment, or sham treatment was used. Mice with untreated tumors required early euthanasia as tumors became too large. Treated tumors were collected 5 and 12 days post treatment start while UNtreated tumors were collected Day 5 and Day 8. Control Day 1 samples (mice euthanized on the start date of treatment) were compared to other groups. Overall design: Radiation, Onalespib or combination treatment induced gene changes to FaDu human xenograft samples grown on athymic mice were collected after 1 or 2 weeks of treatment. Tumor samples were collected and used for RNA microarray. 37 samples collected total, 3 samples minimum per treatment, Control Day 1, Control Week 1, Control Week 2, Drug Week 1 (55mg/kg 5 days per week), Drug Week 2, XRT (radiation, 3 x 2Gy, every other day) Week 1, XRT Week 2 (radiation week 1, no radiation week 2), Drug+XRT Week 1 (1 week radiation, 1 week drug on same schedule as Drug alone and XRT alone), Drug+XRT Week 2 (2 weeks of Drug, 1 week XRT)