Cloning and comparison of human P63+ progenitor cells from various lung diseases [bulkRNA-seq]

KRT5+P63+ progenitor cells located in the basal layer of the airway epithelium have been identified as a promising candidate for lung epithelium repair. To investigate P63+ progenitor cells in human, we cloned KRT5+P63+ progenitors from both healthy donors and patients with various lung diseases. Overall design: Via bronchoscopy, a small amount of 3-5th level bronchi tissue sample were collected from patients. The obtained tissue was washed and enzymatically digested to form a single-cell suspension, which was then cultured under regenerative cell cloning system. In total, we conducted whole-genome RNA sequencing to analyze the transcriptome of P63+ progenitors from 44 donors, including 6 healthy controls and 12 patients with IPF, 13 with COPD, and 13 with non-cystic fibrosis bronchiectasis for comparative analysis.