Computational Analysis of Two-dimensional High-throughput Data from Large-scale RNAi Screens and Single-cell Transcriptomics

This publication provides a singularity definition file to reproduce the computational environment along with the scripts to reproduce every figure or table in the revised manuscript using ZetaSuite Perl module and R package. First, generate a new folder and then download all the files into the folder. Then, uncompressed the files DataSets_part1.tar.gz,DataSets_part2.tar.gz,DataSets_part3.tar.gz,DataSets_part4.tar.gz, and scripts.tar.gz. within the folder. Next, move all the files in DataSets_part1 folder, DataSets_part2 folder,DataSets_part3 folder and DataSets_part4 folder to a new folder called DataSets. Finally, run the following scripts to generate the figures and tables in our manuscript. Regeneration of Figure2 and S2: singularity exec ZetaSuite.sif sh Figure2andS2.sh Regeneration of Figure3 and S3: singularity exec ZetaSuite.sif sh Figure3andS3.sh Regeneration of Figure4 and S4: singularity exec ZetaSuite.sif sh Figure4andS4.sh Regeneration of Figure5 and S5: singularity exec ZetaSuite.sif sh Figure5andS5.sh Regeneration of Figure6 and S6: singularity exec ZetaSuite.sif sh Figure6andS6.sh Regeneration of Figure7 and S7: singularity exec ZetaSuite.sif sh Figure7andS7.sh