ALDH1L1 bacTRAP microarray from striatum 12, 24, and 48 hrs post-MPTP exposure (mice)

Raw data from Illumina MouseRef-8 v2 Expression BeadChip microarray. ALDH1L1 bacTRAP mice were exposed to a single dose of MPTP (12.5 mg/kg, s.c.) or saline and sacrificed at 12, 24, or 48 hours post-exposure. Astrocyte RNA was isolated from pooled striatal tissue (7 mice/sample; 2-3 samples per group) following the TRAP procedure [Heiman et al. (2014) Cell type-specific mRNA purification by translating ribosome affinity purification (TRAP). Nat Protoc, 9: 1292-1291.]

来源于Illumina MouseRef-8 v2 表达 beadChip 微阵列的原始数据。ALDH1L1 bacTRAP 小鼠在接受单剂量MPTP（12.5 mg/kg，皮下注射）或盐水处理后，分别在暴露后12、24或48小时进行处死。随后，采用TRAP程序[Heiman等（2014）通过翻译性核糖体亲和纯化（TRAP）进行细胞类型特异性mRNA纯化。Nat Protoc, 9: 1292-1291]从汇集的纹状体组织中提取星形胶质细胞RNA（每样本7只小鼠；每组2-3个样本）。