Gene expression of depletion of lncRNA Miat in mouse stem-like MB cells [miRNA-Seq]

To investigate the effect of gene alteration of Miat and Mtdh in mouse stem-like MB cells. by canonical oncogenic signaling to maintains the self-renewal capacity of a population of tumorigenic stem-like medulloblastoma cells that are resistant to cytotoxic therapy. by canonical oncogenic signaling to maintains the self-renewal capacity of a population of tumorigenic stem-like medulloblastoma cells that are resistant to cytotoxic therapy. Miat and Mtdh cooperate to promote medulloblastoma tumorigenesis and treatment resistance by regulating the biogenesis of a subset of microRNAs that target genes involved in these processes. Overall design: CRISPR/sgGal4-4 and sgMiat in stem-like MB cells