Molecular Profiling Provides Clinical Insights into Targeted and Immunotherapies as well as Prognosis for Colorectal Cancer. Molecular Profiling Provides Clinical Insights into Targeted and Immunotherapies as well as Prognosis for Colorectal Cancer

We performed single cell sequencing to validate the genomic-guided predictive efficacy of immunotherapy and comprehend the interactions between tumor cells and immune-system cells in cases of wtih complete response and progressive disease. Overall design: Two treatment-naive colorectal cancer patients (one MSI-high/TIB-high and one POLE-mutant/TMB-UH) were recruited and received a single dose of pembrolizumab. Paired endoscopic biopsies of the tumor and adjacent normal tissue were collected for single cell sequencing at baseline and after two courses of neoadjuvant therapy.