Investigating the effects of anti-pseudomonal agents

This dataset presents the investigating of the effects of six primary anti-pseudomonal antibiotics, namely amikacin, cefepime, ciprofloxacin, meropenem, piperacillin and tazobactam, on the planktonic growth of, and formation of biofilm by three different strains of the resilient respiratory pathogen, Pseudomonas aeruginosa [two drug-sensitive strains: the wild-type reference strain, PAO1(WT), and a clinical isolate (DS), as well as a multidrug resistant (MDR) clinically isolated variant of the pathogen].
These agents were investigated individually and in combination with the macrolide antibiotic, clarithromycin. Although all three test strains of P. aeruginosa are resistant to clarithromycin, this agent was included as an adjunct to the conventional anti-pseudomonal agents because of its inhibitory effects on various virulence factors of the pathogen. Following acquisition of the minimal inhibitory concentrations (MICs) of the individual anti-pseudomonal agents with respect to planktonic growth of, and biofilm formation by all three strains of P. aeruginosa, these experiments were repeated using the test anti-pseudomonal antibiotics in combination with clarithromycin. The results showed that amikacin, cefepime, ciprofloxacin and meropenem individually were potent inhibitors of the growth and formation of biofilm of the two susceptible strains of the P. aeruginosa, while, as expected, the MDR strain was highly resistant. When used in combination with clarithromycin, however, synergistic interactions with amikacin, cefepime and ciprofloxacin were observed, while additive activity was observed with the MDR strain.

本数据集旨在探究六种主要抗铜绿假单胞菌抗生素（包括阿米卡星、头孢吡肟、环丙沙星、美罗培南、哌拉西林和他唑巴坦）对耐药性呼吸道病原体铜绿假单胞菌三种不同菌株的浮游生长及生物膜形成的影响。其中，两种菌株为药物敏感型（野生型参考菌株PAO1(WT)及临床分离株DS），另一种为多药耐药型（MDR）的临床分离株。这些抗生素分别及其与宏量霉素类抗生素克拉霉素的联合使用均进行了研究。尽管三种铜绿假单胞菌测试菌株对克拉霉素具有耐药性，但由于其对病原体多种致病因子具有抑制作用，因此将其作为传统抗铜绿假单胞菌药物的辅助用药。在获得针对三种铜绿假单胞菌菌株的浮游生长及生物膜形成的单一抗铜绿假单胞菌抗生素的最小抑菌浓度（MICs）后，这些实验再次使用测试抗生素与克拉霉素的联合使用进行了重复。结果表明，阿米卡星、头孢吡肟、环丙沙星和美罗培南单独使用时对两种易感型铜绿假单胞菌的生长和生物膜形成具有显著的抑制作用，而多药耐药菌株对它们具有高度耐药性。然而，当与克拉霉素联合使用时，观察到阿米卡星、头孢吡肟和环丙沙星之间存在协同作用，而对多药耐药菌株则表现出相加活性。