The presence of MeCP2 associated factors at the amplified chromosomal array upon MeCP2 targeting.

Various factors were assayed at light microscopical level for their presence at the amplified chromosomal array in the AO3_1 clone (CHO derived clone containing an amplified chromosomal region consisting of the DHFR cDNA transgene and 256 lac operator repeats). Immunolabeling or co-transfection were performed upon expressing EGFP-lacR (control) or EGFP-lacR-MeCP2. Localization at the array is scored as (+) present, (+/−) infrequently present or (−) absent.