Punic people were genetically diverse with almost no Levantine ancestors

The maritime Phoenician civilization from the Levant transformed the entire Mediterranean during the first millennium BCE. However, the extent of human movement between the Levantine Phoenician homeland and Phoenician-Punic settlements in the central and western Mediterranean has been unclear in the absence of comprehensive ancient DNA studies. We generated genome-wide data for 210 individuals, including 196 from 14 sites traditionally identified as Phoenician and Punic in the Levant, North Africa, Iberia, Sicily, Sardinia, and Ibiza, and an early Iron Age individual from Algeria. Levantine Phoenicians made little genetic contribution to people living in Punic settlements in the central and western Mediterranean between the 6th and 2nd centuries BCE, despite abundant archaeological evidence of strong cultural, historical, linguistic, and religious links. Instead, these inheritors of Levantine Phoenician culture derived most of their ancestry from populations with a genetic profile similar to that of Sicily and the Aegean. Much of the remaining ancestry originated from North Africa, reflecting the growing influence of Carthage; however, this was a minority contributor of ancestry in all sampled sites, including in Carthage itself. Different Punic sites across the central and western Mediterranean show similar patterns of high genetic diversity. We also detect genetic relationships across the Mediterranean, reflecting shared demographic processes shaping populations around the Punic world.