Diversity and Signature of Small RNA in Different Bodily Fluids using Next Generation Sequencing

Small RNAs are critical components in regulating various cellular pathways. These molecules, which could be tissue-associated or circulating in bodily fluids, have been shown to associate with different tumors. Next generation sequencing on small RNAs is a powerful tool for profiling and discovery, particularly for microRNA (miRNA).In this study, we isolated total RNA from various bodily fluids (blood, leukocytes, serum, plasma, saliva, cell-free saliva, urine and cell-free urine). Then, Illumina’s next generation sequencing was used to investigate the distribution and signature of small RNAs in these fluids through intensive bioinformatics analysis performed on the sequencing data.