Identification of renal resident macrophages across species [C1]

We use single cell RNA sequencing to identify an evolutionarily conserved gene expression signature in resident macrophages. Based on our single cell data, we show the presence of a core gene expression signature in a cluster of innate immune cells in mouse, rat, pig, and human kidney tissue. Further, using this gene expression signature, we identify novel candidate markers for resident macrophages that span all 4 species tested. Overall design: We performed 10x genomics and fluidigm C1 single cell sequencing on innate immune cells isolated from the kidney of mouse, rat, pig, and human kidney tissue. This series includes mouse and human samples.