Processed RNA-sequencing data and analysis documentation for Caco-2 cells and CT26-derived tumors

This dataset contains processed RNA-sequencing data and supporting documentation for human Caco-2 cells treated in vitro and CT26-derived mouse tumor tissues collected following in vivo treatment. The Caco-2 dataset includes four groups: saline, MR@Ir, NTMR@Ir, and NTMR@Ir+M. The CT26 tumor tissue dataset includes four groups: Saline, Drug, NTMR@Ir, and NTMR@Ir+M. Each experimental system includes three independent biological replicates per group, totaling 12 samples per system and 24 samples overall. The human and mouse datasets were analyzed separately. Files are organized into separate directories for the two experimental systems: For CT26 tumor tissues, “AnnotationExpress” contains gene-level count and TPM expression matrices; “Background” contains sample names, treatment groups, corresponding SRA run accessions, and reference genome information; “DiffExpress” contains differential expression analysis materials corresponding to the volcano plots, Venn diagrams, and related analyses reported in the manuscript; “GenesetEnrich” contains GO and KEGG enrichment analysis results reported in the manuscript; and “software” contains information on the analysis software used. The Caco-2 directory contains the corresponding “AnnotationExpress”, “Background”, “GenesetEnrich”, and “software” folders. The corresponding raw sequencing data have been deposited in the NCBI Sequence Read Archive (SRA) under BioProject accession PRJNA1530467: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1530467.