DArTseq genotypes for koalas across south-western Sydney and the Southern Highlands, Australia

Genotype data for the paper: "Low genetic diversity and high inbreeding in one of the last chlamydia-free strongholds for New South Wales koalas" published in Conservation Genetics. 

Koala samples were collected across south-western Sydney and the Southern Highlands in Australia and sequenced using DArTseq (Diversity Arrays Technology PL, Canberra), a form of reduced representation seqencing across four sequencing runs. DNA samples were digested using the enzyme combination PstI-SphI and fragments were sequenced on a HiSeq 2500 or NovaSeq 6000 as 77-138 base pair single-end reads.

These genotypes are the result of DArT's proprietary pipeline of SNP markers, both aligned to the koala reference genome (GCA_002099425.1_phaCin_unsw_v4.1) and assembled de novo. Information columns include the reference allele, alternate allele, genomic position, call rate, average reproducibility (calculated as consistency in marker score between technical replicates performed by DArT [N = 91]), and the frequency of homozygotes and heterozygotes for 23,384 SNPs across 111 koalas.

Funding for this study was provided by the New South Wales Government Office of the Chief Scientist and Engineer for the Koala Genome Survey.