Expression data from mouse atrial Angiotensin II treated for 0,1,2,3 weeks

AF is characterized by structural and electrical remodeling, including atrial enlargement, fibrosis, inflammation, and ion-channel alteration, which frequently results in arrhythmia recurrence and maintenance.However, the gene expression profiles signaling pathways in the atria during the development of AF induced by Ang II remain unknown All mice were infused with Ang II (2000 ng/kg/min) using an osmotic pump for 1, 2 and 3 weeks. At the end of Ang II infusion, intracardiac pacing was performed in these animals by inserting an eight-electrode catheter (1.1 F, octapolar EP catheter, Science) through the jugular vein and advancing it into the right atrium and ventricle. All mice were euthanized by an overdose of pentobarbital on weeks 1, 2 and 3 of Angiotensin II treatment. Total RNA was isolated with TRIzol (Invitrogen) from atrials (n=4 per group) at each time point according to manufacturer’s instructions. Gene expression profiling was performed using Affymetrix GeneChip mouse Genome 430 2.0 array according to the manufacturer’s instructions (Affymetrix, Inc., Santa Clara, CA). On the GeneChip Mouse Genome 430 2.0 Array, over 45,000 probe sets analyze the expression level of over 39,000 transcripts and variants from over 34,000 well characterized mouse genes.