The role of Macrophage-inducible C-type lectin (Mincle) signaling in Crohn's disease

Macrophage-inducible C-type lectin (Mincle) signaling plays a proinflammatory role in different organs such as the brain and liver, but its role in intestinal inflammation remains unknown. We studied the characteristics of Mincle signaling expression in CD patients and experimental colitis. The functional role of Mincle signaling in the intestine was addressed in experimental colitis models in vivo by using mice with Mincle knock out (Mincle-/-), neutralising anti-Mincle antibody, downstream Syk inhibitor, Mincle pharmacologic agonist and RNA-seq genome expression analysis. Bone marrow-derived macrophages were collected from mice and used to further verify the effect of Mincle signaling in macrophages.