Discordant regulation of eIF2 kinase GCN2 and mTORC1 during nutrient stress

Here we report Total RNA seq and RiboSeq on MEF cells treated with halofuginone. Halofuginone is a competitive inhibitor of the Prolyl-tRNA synthetase that results in an accumulation of uncharged Prolyl-tRNA upon treatment that leads to an activation of the protein kinase Gcn2. Overall design: Total RNA Seq and ribosome footpinting in halofuginone and untreated MEF cells