Scalable purification of cowpea chlorotic mottle virus (CCMV) and its bioconjugates from plant extracts using ultrafiltration

Plant virus-derived materials are finding applications in science, engineering, and technology – with some candidates advancing toward commercialization, scalable manufacturing methods are needed. We recently established ultrafiltration/diafiltration (UF/DF) for purification of cowpea mosaic virus (CPMV) and tested here broader applicability of this process by purifying cowpea chlorotic mottle virus (CCMV) and its bioconjugates. We optimized extraction, ultrafiltration and ion exchange chromatography for CCMV: acidic buffers (pH 4.0) were ideal for extraction, removing ~80% of plant host cell proteins while recovering nearly 100% of CCMV. Membranes with a 1000 kDa and 300 kDa molecular mass cut-off were best suited for ultrafiltration, allowing to remove impurities, both larger and smaller than CCMV, alongside bulk water. When combining these steps, the same level of purity (>99%) as the contemporary centrifugation process was achieved with only 7 instead of 13 process steps, thus sh..., , # Scalable purification of cowpea chlorotic mottle virus (CCMV) and its bioconjugates from plant extracts using ultrafiltration

Dataset DOI: [10.5061/dryad.1c59zw49x](https://doi.org/10.5061/dryad.1c59zw49x)

## Description of the data and file structure

The following files contain the raw datasets required to reproduce the analyses presented in the article *“Scalable purification of cowpea chlorotic mottle virus (CCMV) and its bioconjugates from plant extracts using ultrafiltration\". *This article was authored by Patrick Opdensteinen, Kyle Lewin, and Nicole Steinmetz (Aiiso Yufeng Li Family Department of Chemical and Nano Engineering, University of California, San Diego, CA, USA) for *Biotechnology Journal* (2026).

These datasets support a comparison of two methods for plant virus purification: ultrafiltration and centrifugation. Cowpea chlorotic mottle virus (CCMV) was extracted from infected host plant material using each purification method. The resulting viral protein ..., ,