m1A enrichment analysis of SNU-668 Erastin-resistant cells transfected with shRNA-ALKBH3 or control shRNA

An m1A enrichment analysis was conducted to investigate the epigenetic landscape and RNA modification profiles within SNU-668 Erastin-resistant gastric cancer cells following the experimental manipulation of ALKBH3 expression. Specifically, this analysis compared cells that had been stably transfected with a targeted shRNA construct designed to knock down ALKBH3 against control cells transfected with a non-targeting scramble shRNA sequence. The overarching goal was to assess and quantify the differential enrichment patterns of N1-methyladenosine (m1A) modifications across the transcriptome, thereby elucidating the potential regulatory role of the ALKBH3 demethylase in modulating the epitranscriptomic state underlying the acquired Erastin-resistant phenotype in this cellular model. Overall design: MeRIP m1A enrichment analysis of SNU-668 Erastin-resistant cells transfected with shRNA-ALKBH3 or control shRNA