High-resolution mapping and transcriptional profiling of coronary-cardiomyocyte interactions during zebrafish heart development

We used a 10X Genomics single-cell RNA sequencing approach to study transcriptional profiling of coronary developmental stages in zebrafish comparing data from initiation (10 mm), establishment (14 mm), expansion (18 mm) and maturation (28 mm). Overall design: One pool of ventricles per developmental stage, initiation (10 mm) 25 ventricles, establishment (14 mm) 18 ventricles, expansion (18 mm) 12 ventricles and maturation (28 mm) 5 ventricles were extracted for single-cell dissociation and library preparation.