Mechanism of DNA unwinding by MCM8-9 in complex with HROB|DNA解旋数据集|生物化学数据集

HROB promotes the MCM8-9 helicase in DNA damage response. To understand how HROB activates MCM8-9, we defined their interaction interface. We showed that HROB makes important yet transient contacts with both MCM8 and MCM9, and binds the MCM8-9 heterodimer with the highest affinity. MCM8-9-HROB prefer branched DNA structures, and display low DNA unwinding processivity. MCM8-9 unwinds DNA as a hexamer that assembles from dimers on DNA in the presence of ATP. The hexamer involves two repeating protein-protein interfaces between the alternating MCM8 and MCM9 subunits. One of these interfaces is quite stable and forms an obligate heterodimer across which HROB binds. The other interface is labile and mediates hexamer assembly, independently of HROB. The ATPase site formed at the labile interface contributes disproportionally more to DNA unwinding than that at the stable interface. Here, we show that HROB promotes DNA unwinding downstream of MCM8-9 loading and ring formation on ssDNA., Here, we present mass photometer data and single-molecule magnetic tweezer data from the main and supplementary figures of the manuscript, \"Mechanism of DNA unwinding by MCM8-9 in complex with HROB\". The files uploaded include all the information needed to plot the panels as indicated in the attached main and supplementary figures and the file names.
We share information obtained through mass photometry movies and single-molecule magnetic tweezers techniques to measure DNA unwinding, featured in both the main and supplementary figures of the study, \"Mechanism of DNA unwinding by MCM8-9 in complex with HROB\".
Mass photometry measurements were performed on a 2MP-0132 mass photometer (Refeyn). Sample binding to the coverslip surface was monitored by recording a movie for 1 minute using AcquireMP (Refeyn Ltd) software. Data analysis was performed using DiscoverMP (Refeyn Ltd). For each peak analyzed, at least 500 events were considered. To convert the measured optical reflection-interferenc..., , # Mechanism of DNA unwinding by MCM8-9 in complex with HROB

[https://doi.org/10.5061/dryad.wdbrv15wq](https://doi.org/10.5061/dryad.wdbrv15wq)

Here, we present mass photometer data and single-molecule magnetic tweezer data from the main and supplementary figures of the manuscript, \"Mechanism of DNA unwinding by MCM8-9 in complex with HROB\". The files uploaded include all the information needed to plot the panels as indicated in the attached main and supplementary figures and the file names.

## Description of the data and file structure

We share information obtained through mass photometry and single-molecule magnetic tweezers techniques to measure DNA unwinding, featured in the study's main and supplementary figures.

## Sharing/Access information

Data was derived from the following sources:

* Mass photometry measurements were performed on a 2MP-0132 mass photometer (Refeyn). Sample binding to the coverslip surface was monitored by recording a movie for 1 minute using AcquireMP ...