Replicative history marks transcriptional and functional disparity in the memory T cell pool II

To validate whether division history is linked to functional and transcriptional properties we established a CTV-based serial-transfer approach that allowes single cell transcriptional profiling of memory T cells that have undergone a high or low amount of prior division Naïve OT-I and GFP;OT-I T cells were transferred into primary recipients that were subsequently exposed to Lm-OVA infection. At day 30 post-infection, early memory T cells were harvested, CTV labeled and transferred into infection-matched secondary recipients. 75 days later, CTVHI (div0-2) and CTVLO (div5+) TCM were isolated from the GFP+ and GFP- pools, and the resulting TCM populations were then profiled by scRNAseq.