Direct and indirect targets of Arabidopsis ABI3: genome wide binding sites [ChIP-chip]

ABSCISIC ACID INSENSITIVE3 (ABI3) is a transcription factor that is a member of the B3 domain family and is involved in seed development. To understand how it controls development, we mapped genome-wide binding sites by ChIP-chip and combined this data with transcriptomic data obtained by microarrays. Ran Tian, Fangfang Wang, Qiaolin Zheng, Venus Niza, A. Bruce Downie and Sharyn E. Perry (2020) ABI3 was expressed by its native promoter and included a C-terminal 10x c-myc tag. This transgene was able to rescue the abi3-5 mutant. A second transgene (35S:AGL15) allowed establishment of stable somatic embryo cultures. ChIP was performed using anti-c-myc antibody (Myc-Tag (9B11) Mouse mAb; Cell Signaling 2276S) on tissue expressing ABI3-myc (three biological replicates) and on three controls of tissue without c-myc antibody.